DNA Extraction and Purification
New England Biolabs, Inc. Monarch® RNase A - 1 ml - (2 x 0.5ml)
Monarch RNase A, a component of the Monarch Genomic DNA Purification Kit, can be used to purify genomic DNA from a variety of sample types. It is used at 56°C in the kit protocol but is active at any temperature 20-65°C. Treatment with RNase A is an optional step in the Monarch Genomic DNA Purification Kit protocol for removal of any residual RNA. Co-purification of RNA during gDNA extractions is a common problem that leads to the overestimation of DNA yield. The low alcohol binding conditions employed in the kit optimizes binding of gDNA alone, so RNA co-purification is extremely low.
- Monarch RNase A degrades single-stranded RNA at C and U residues
- Purified from cow pancreas and is free of contaminating protease or DNase activity
- Contains no residual host DNA
- It is supplied as a 20 mg/ml solution in 50% glycerol, 50 mM Tris-Cl pH 7.4
- Monarch kit components are also available separately for added convenience
Electron Microscopy Sciences NSA (Nonenyl Succinic Anhydride) 450 ML
F.W. 227
Boiling point: 325 °C (617 °F)
An epoxy hardener that is specially distilled. The special distillation process offers you much clearer blocks than the standard NSA
Macherey-Nagel NucleoSpin Food, 250 preps
NucleoSpin Food (250) 250 preps for the isolation of genomic DNA from food - NucleoSpin Food Columns, Collection Tubes, buffers, Proteinase K
Macherey-Nagel MACHEREY-NAGEL NucleoSpin RNA Kit, High-Yield RNA Isolation, On-Column rDNase Digest, 250 Preps
The MACHEREY-NAGEL NucleoSpin RNA Kit is an efficient RNA isolation kit that facilitates the purification of high-quality RNA from various sample types, including cells and tissues. With an included on-column rDNase for DNA removal and quick preparation time, it streamlines workflows for sensitive applications like RT-PCR and RNA-sequencing. Its silica membrane technology ensures reliable results, making it a standout choice for scientists needing effective and dependable RNA purification.
Macherey-Nagel NucleoBond PC 500, 25 preps
NucleoBond PC 500 (25) 25 preps for the isolation of plasmid DNA - NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A
Research Products International Corp ZR Plasmid Miniprep-Classic Kit (400 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
New England Biolabs, Inc. NEBNext Ultra™ II FS DNA Library Prep with Sample Purification Beads – 24 reactions
The NEBNext Ultra II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, the NEBNext Ultra II Q5 Master Mix for uniform, high-fidelity library amplification, and the gold standard SPRIselect size selection and clean-up beads. Please note that adaptors and primers are not included in the kit and are available separately.
Edge Biosystems Inc PERF. DTR ULTRA 96-WELL PLT KT
fully hydrated gel filtration matrix that provides for a convenient and simple method of dye terminator removal for sequencing cleanup.
Zymo Research Corporation ZymoBIOMICS™ MagBead (96 preps)
The provided DNA/RNA Shield™ inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method utilizes magnetic beads for DNA/RNA extraction without the use of phenol and is eluted into 50 l of ZymoBIOMICS™ DNase/RNase-Free Water. RNA is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
Zymo Research Corporation DNA Clean & Concentrator™-5 (200 Preps) w/ Zymo-Spin™ I Columns (Uncapped)
It can also be used for post-RT cDNA clean-up andpurification of sequencing-ready DNA from M13 phage. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ Column. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin column technology to yield DNA that is free of salts and contaminants in just 2 minutes. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures.
Research Products International Corp ZymoPURE II Plasmid Maxiprep, 10 Preps
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready.