DNA Extraction and Purification
New England Biolabs, Inc. Monarch® HMW DNA Extraction Kit for Tissue - 5 preps
The Monarch HMW DNA Extraction Kit for Tissue provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from various tissues and bacteria, as well as other sample types including yeast, insect, and amphibian. Optimized extraction protocol for tissue utilizes pestle homogenization and proteinase K digestion with agitation for sample lysis, followed by a protein removal step and precipitation of the extracted DNA onto the surface of large glass beads. DNA size ranges from 50 to greater than or equal to 500 kb for standard protocols and into the Mb range when the lowest agitation speeds are used for processing soft organ tissues and bacteria. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. Purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing
New England Biolabs, Inc. Monarch® HMW gDNA Tissue Lysis Buffer - 62 ml
The Monarch HMW gDNA Tissue Lysis Buffer, supplied as a component of the Monarch HMW DNA Extraction Kit for Tissue, is a highly effective lysis buffer for tissue and bacteria samples. Its composition ensures immediate and complete inactivation of nucleases in the samples at the start of the lysis.
- Supplied as a component of the Monarch HMW DNA Extraction Kit for Tissue
- This lysis buffer enables rapid and effective lysis of various tissue and bacteria samples
- Offers maximal protection against nucleases allowing for the isolation of intact HMW DNA
- Supplied as one bottle of 62 ml
Biotium AccuGreen™ High Sensitivity dsDNA Quantitation Kit (100 assays)
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit fluorometers from Thermo Fisher. Unlike absorbance-based measurements, AccuGreen™ Dye is highly selective for double-stranded DNA over single-stranded DNA or RNA. The assay has a green fluorescence with an excitation/emission of 502/523 nm (with DNA). The assay has a linear range of 0.1-100 ng dsDNA and a sample concentration range between 5 pg/uL and 100 ng/uL. This trial size kit has sufficient reagent for 100 assays.
Roche Diagnostics KAPA Express Extract Kit | Standard Kit, 200u
KAPA Express Extract Kit is a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA in as little as 15 minutes. DNA extractions are conveniently performed in a single-tube thereby greatly reducing the risk of sample loss and contamination. *This item is non returnable.
Roche Diagnostics KAPA HYPERPLUS KITS W/LBRY APP
KAPA HYPERPLUS KITS W/LBRY APP *This item is non returnable.
Bioworld Glass Milk, 5 mL
Glass Milk Specifications:Glass Milk is a specially prepared suspension of propriety silica matrix in an aqueous solution used to isolate and purify DNA. Provides rapid, quantitive, specific binding of DNA/RNA and is able to clean up DNA from solution or clear-up gel bands. DNA purified by Glass Milk shows no effect on sequencing reactions, restriction digests, ligations or phosphorylations. Also suitable for mRNA preparation. Can be stored and shipped at room temperature
Macherey-Nagel Collection Tubes (1000)
Collection Tubes (1000) Collection Tubes 2 mL, without lid pack of 1000
Zymo Research Corporation EZ-96 DNA Methylation-Gold™ Kit (Deep-Well) (2 x 96 Rxns)
The EZ-96 DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation instead of chemical denaturation which is used in the original EZ DNA Methylation™ Kit. Also, the EZ DNA Methylation™-Gold™ Kits have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation Quick-DNA™ Miniprep Plus Kit (200 Preps)
The Quick-DNA™ Miniprep Plus Kit is the easiest method for high yield total DNA extraction (e.g., genomic, plasmid, mitochondrial, viral) from any biological fluid,cell culture, or solid tissue sample. Innovative reagents and Zymo-Spin™ Technology allow for ultra-pure and concentrated genomic DNA 50 kb to be eluted in as little as 35 l. Zymo-Spin™ Columns ensure no buffer retention. Purified DNA is RNA-free, bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
![Zymo Research Corporation ZymoBIOMICS DNA Microprep Kit (50 preps)Includes: [D4305] ZymoBIOMICS Microprep Kit x 1 [S6012-50] ZR BashingBead Lysis Tubes 50 pack x 1[D4300-1-40] ZymoBIOMICS Lysis Solution, 40 ml x 1](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D4301ZYMOBIOMICSDNAMICROPREPKIT.JPG-250.jpg)
Zymo Research Corporation ZymoBIOMICS DNA Microprep Kit (50 preps)Includes: [D4305] ZymoBIOMICS Microprep Kit x 1 [S6012-50] ZR BashingBead Lysis Tubes 50 pack x 1[D4300-1-40] ZymoBIOMICS Lysis Solution, 40 ml x 1
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Zymo Research Corporation ZymoPURE Plasmid MiniPrep Kit (10 Preps)
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 g/l).In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Agilent Technologies RECOVEREASE DNA ISOLATION KIT
RecoverEase DNA Isolation Kit recovers high-molecular-weight (100 to 500 kb) genomic DNA from whole tissue. It is ideal for DNA isolation for transgenic mutation assays.No organic extractions or ethanol precipitations are required.
Zymo Research Corporation Zymo Spin™ III-P Column Assembly (5 Pack) w/ 15 ml and 50 ml Reservoir
The Zymo-Spin III-P features durable polypropylene construction and a unique silica-based matrix that allows for purification of up to 300 g of plasmid DNA in 100 l eluate when used in combination with ZymoPURE Plasmid Prep buffers. Capacity with reservoir assembly is 65 ml.
![Zymo Research Corporation Zymoprep™ -96 Yeast Plasmid Miniprep (2 x 96 preps) [Includes E1004 x 2: Zymolyase (1 x 2000 Units Lyophilized) w/ Storage Buffer (500µl)]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D2005ZYMOPREPYEASTPLASMIDMINI.JPG-250.jpg)
Zymo Research Corporation Zymoprep™ -96 Yeast Plasmid Miniprep (2 x 96 preps) [Includes E1004 x 2: Zymolyase (1 x 2000 Units Lyophilized) w/ Storage Buffer (500µl)]
The Zymoprep™-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep™-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase™ added in the first solution. There is no need for glass beads, or phenol.